RESUMO
Inula helenium roots are consumed as natural flavor components and raw or cooked as food, and their extracts are rich in sesquiterpene lactones such as alantolactone (AL) and isoalantolactone (IAL), which have recently attracted great attention due to their pharmacological properties. The industrial utilization of these compounds requires the development of green, efficient, cost-effective, and sustainable extraction protocols. Therefore, this study focused on the optimization of microwave-assisted extraction (MAE) process variables using Face-Centered Central Composite Design (FC-CCD). Then, maceration was applied as a conventional technique, and these techniques were compared in terms of extraction efficiency, morphological changes, antimicrobial activities, carbon emissions, and energy consumption. As a result, optimal MAE conditions, i.e., EtOH: water ratio (X 1) = 100:0, liquid/sample ratio (X 2) = 30:1 mL/g, microwave power (X 3) = 300 W, and irradiation time (X 4) = 5 min, were obtained with AL and IAL yields of 54.99 ± 0.11 (mg/g) and 48.40 ± 0.19 (mg/g), respectively. The extract obtained by MAE had similar or better activity than positive controls in most cases and formed the largest inhibition zones against E. coli (29.5 ± 0.71 mm) and A. niger (34.75 ± 1.06 mm). Morphological changes of I. helenium roots after extraction were observed by scanning electron microscopy. Additionally, MAE was 43.4 times faster than maceration, resulting in 228.6 times less energy consumption and carbon emissions. Based on these findings, it is recommended to use MAE as an industrial green technique for the extraction of sesquiterpene lactones with potential applications in nutraceuticals and food products in terms of sustainable economy and environmental protection.
RESUMO
Klasea is a taxonomically complex genus in which there are many problems, mostly with Klasea kotschyi and Klasea hakkiarica. It is challenging to differentiate the genera based on morphological characters alone. Revision studies performed on the basis of molecular data obtained from studies conducted in recent years have made the phylogenetic relationships and systematic positions of the taxa more apparent and reliable. In this study, Klasea, Serratula, Jurinea and Centaurea species native to Turkey, were collected from different localities of Anatolia and DNA was isolated from the collected samples. The data were analyzed ordination analyses including UPGMA and PCA using NTSYSpc 2.1. The infrageneric and intergeneric phylogenetic relationships between Klasea and other related genera were also characterized. The Klasea species were grouped into three clusters. It was determined that taxa Klasea kotschyi and Klasea hakkiarica are separate but closely related. Moreover, it was observed that the Klasea lasiocephala a separate group within the genera. Clearly the genera Klasea, Serratula, Jurinea and Centaurea are phylogenetically differentiated on the dendogram.
RESUMO
A new subspecies Seseli gummiferum Pall. ex Sm. subsp. ilgazense A.Duran, Ö.Çetin & M.Öztürk, subsp. nov. (Apiaceae) is described from Kastamonu province, Turkey. It was collected from the open Pinus sylvestris L. and Abies nordmanniana (Steven) É.Spach. mixed forest in the northern Anatolian region. An endemic apparently confined to the Ilgaz Mountain National Park, the new taxon is closely related to Seseli gummiferum subsp. gummiferum. Diagnostic morphological characters for closely similar taxa are discussed, and a key to the subspecies of Seseli gummiferum is presented. ITS (Internal Transcribed Spacer) region of the nuclear ribozomal DNA of closely related Seseli L. taxa and Pimpinella is used to constract phylogenetic tree by using BioEdit and Seaview Programme.
